Key Words: tangential flow filtration, graphene oxide, protein, enzyme, antibody, metal ligands
The IMAC technology, developed over 40 years ago,(6) has long been envisioned as the most effective low-cost solution for addressing all of these needs, but high hopes were dimmed by significant drawbacks(2, 3) for which no practical remedies are available: mainly metal ion leaching, size-variant protein capacity, and denaturation of some proteins. Contrary to common belief, the need to cleave the epitope-tag in pharmaceutical applications has not been the reason for the limited success of IMAC resins.(3) While GO-based resin in conjunction with new metal coordination ligands hold great promise in solving these issues they are still incompatible with large columns. A revisited version of the TFF method can provide a pathway for large scale use of these resins, with greatly increased market opportunity.
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