IDEA #1PS3YT TUBERCULOSIS EARLY DIAGNOSTICS BY NANOTEÑHNOLOGICAL ELECTROCHEMILUMINESCENT SENSOR

TUBERCULOSIS EARLY DIAGNOSTICS BY NANOTEСHNOLOGICAL ELECTROCHEMILUMINESCENT SENSOR Fig. 1. Structure of semiconductor quantum dot Fig. 2. ECL sensor work electrode: sketch Fig.3. Stage of ECL detection in tuberculosis diagnos- tic Stage of Development Development phase – technology and instrument labora- tory tested. Contact Details Mykola Rozhitskii, Professor, Dr. of Phys.-Math Sci., Academician of Applied Radio Electronics Academy, Scientific Supervisor of Laboratory of Analytical Opto- chemotronics. Olena Galaichenko, PhD, Senior Researcher. Address: Ukraine, 61166, Kharkiv, 14, Lenin Ave., Kharkiv National University of Radio Electronics, Bio- medical Electronics Dep., Laboratory of Analytical Opto- chemotronics phone: +380 57 7020-369 fax: +380 57 7020-107 e-mail: rzh@kture.kharkov.ua web: http://electrohim.kture.kharkov.ua It main of (TB) diagnostics. several Description The essence of our proposal is novel photonic sensor instrument aimed for assay of bioliquids for early is based on tuberculosis combination ideas: electrochemiluminescent (ECL) detection method; nanotechnological ECL sensor which electrodes coated by molecular thin films doped with reactants quantum dots, “tuned” for tuberculosis markers interception with analytical signal emittance. ECL assay method possesses a) high sensitivity due to optical detection of signal that allows reaching single- photon detection limit, b) high selectivity of the assay due to used quantum dots with different properties and c) two-channel registration mode, i.e. simultaneous recording of electrochemical and optical ECL signals. Incorporation of quantum dots (QD) of different di- mensions as luminescer – reactants into film deposited onto electrode allows us to determine different TB markers (organic molecules A of middle mass) in bioprobes such as blood serum. The high-technology method used for thin films formation and deposition onto working electrodes of the proposed sensor is Langmuir-Blodgett one. It allows formation of uniform ordered nanostructured molecular ensembles with highly controllable predefined structure from semicon- ductor QD. The proposed sensor is aimed at detection of certain TB mycobacterium organic markers in bioliquids (blood serum) important for early recognition of tuber- culosis. Innovative Aspects Proteolysis activation result in accumulation of a con- siderable quantity of products of protein degradation. Some of mentioned substances are unique and can be used as markers of any tuberculosis forms, irrespective of its strain and genetic typing. The sensor’s analytical signal is free from background noise caused by false interaction due to peculiarities of ECL detecting me- thod. The full control over the assay procedure allows introducing automation of diagnostics process in a large number of investigations, including flow analysis principle. Main Advantages - Utilization of novel nanotechnology in combination with electrochemiluminescent detection method; - early tuberculosis diagnostics; - diagnostics of any tuberculosis active and latent forms; - fast and simple assay procedure; - simple sample preparation (~ 1-2 minutes, requires only one person); - low sample volume (~20 μl); - low assay cost. Areas of Application - Medicine; - food industry; - pharmacology; - ecology.