IDEA #9VABN5 Silica_Based, Biologically Active Admixtures To Cryoprotection Media Aimed At Increasing Viability And Longevity Of Post_Thaw Gametes In Medicine And Livestock Breeding

NANOTECHNOLOGY • APPLICATIONRESEARCH IINN THEFIELD OOFF NANOTECHNOLOGYSILICA(cid:2)(cid:2)BASED,, BIOLOGICALLY ACTIVE ADMIXTURES TTOO CRYOPROTECTION MEDIA AIMED AATT INCREASING VIABILITY AND LONGEVITY OOFF POST(cid:2)(cid:2)THAW GAMETES IINN MEDICINE Description AND LIVESTOCK BREEDING Longevity, hours Development of novel applications of nano(cid:2)structured silica in medicine, biotechnology and environmental protection, is a primary focus at the Institute of Sur(cid:2)face Chemistry. Fumed sil(cid:2) ica, when included in cell suspensions, demonstrates high adhesion to the cell surface. Due to interaction with certain terminal car(cid:2)bohydrates of cell membrane receptors, high(cid:2)sur(cid:2) face(cid:2)area fumed silica nano(cid:2)particles localize at select sites of the cell membrane. As a result, nano(cid:2)particles can stimulate or inhibit functional processes in living cells, depending on the silica concentration in the cell sus(cid:2)pension. Moreover, chemi(cid:2) cal and adsorption modifica(cid:2)tion of the surface of nano(cid:2)parti(cid:2) cles by biologically active molecules diversify the spectrum of possible effects of nano(cid:2)structured silica. In particular, carbo(cid:2) hydrate molecules are promising, since they are recog(cid:2)nized by cell receptors. Innovative Aspects and Main Advantages • Nano(cid:2)size fumed silica, used as a carrier in pro(cid:2)posed nano(cid:2)materials, was proved to provide itself the positive bio(cid:2) logical effect with respect to reproductive cells • Biological effect may be optimized by variation of admixture concentration and structure of biomolecules used as modi(cid:2) fiers of the surface • No necessity exist to change the composition of existing and already used cryo(cid:2)media • Synthesized nano(cid:2)materials are used as low con(cid:2)centra(cid:2) tion (less than 0.5%) admixtures to existing cell cryo(cid:2)media. • The price of artificial insemination may be re(cid:2)duced essen(cid:2) tially by reduction of sperm(cid:2)doze number Areas ooff Application • Genetics of reproduction • Animal breeding • Biotechnology Sample # Fig.1 Longevity of bovine gametes after freezing(cid:2)thawing cycle (hours): (1) – native cell medium; (2) – cell medium after admixture of fumed silica; (3(cid:2)8) – cell medium after admixture of fumed silica modified by various biomolecules. Stage ooff Development New silica(cid:2)based nano(cid:2)materials as admixtures to existing cryo(cid:2)media were synthesized and successfully tested with post thaw bovine sperm and human reproductive cells to increase their viability, longevity and level of artificial insemi(cid:2) nation. The development of new materials was realized in the framework of STCU Project # 3103. Product was field tested on animal sperm, was experi(cid:2)mental(cid:2) ly tested on human gametes and is available for demonstra(cid:2) tion. Contact Details Institute of Surface Chemistry, National Academy of Science of Ukraine Contact person: Galagan Natalia Address: 17 Gen. Naumova Str., Kiev, 03164, Ukraine Tel.: (380(cid:2)44) 422 9685 Fax: (380(cid:2)44) 424 3567 E(cid:2)mail: ucs@ln.ua INNOVATIVETECHNOLOGY OPPORTUNITIES FROM THESTCU 159