IDEA #4LUVM7 Miniature Biosensor For Detection Of Glucose And Lactate In Blood

LIFE SCIENCES • MEDICALRESEARCH MINIATURE BIOSENSOR FOR DETECTION OOFF GLUCOSE AND LACTATE IINN BLOOD Description It is proposed bionanotechnology to create a sensitive low(cid:2) cost biosensor for glucose and lactate level detection in blood, based on the carbon nanotubes emission. This technology uti(cid:2) lizes the NIR luminescence of semiconducting single(cid:2)walled carbon nanotubes bound with recognizing biosystems (enzymes: glucose oxidase, lactate oxidase). For this purpose using small diameter nanotubes (0.6(cid:2)1.1 nm) are preferable because its emission can be detected in the spectral range 0.9(cid:2)1.2 mkm by a conventional low(cid:2)cost CCD matrix or photo(cid:2) multiplier. In this spectral range human tissues are optically transparent and a sensor can be implanted into the body. The central issue in designing and forming such devices is a cre(cid:2) ation of the interface between the nanotube and the recogni(cid:2) tion biosystem. To avoid loss of intrinsic nanotube properties upon chemical modification, a noncovalent nanotube func(cid:2) tionalization will be applied. In this R&D we propose two approaches to the functionalization of small diameter nan(cid:2) otubes. First, a chemical compound whose flat part couples with the nanotube surface by means of the р(cid:2)р interaction and with the linker immobilized by the enzyme will be identified and synthesized. Another possible approach to the non(cid:2)cova(cid:2) lent nanotube functionalization is using polymer wrapping around the tube. A method of enzyme immobilization on poly(cid:2) mer(cid:2)wrapped nanotube will be developed. Distinctive features of proposed R&D are: • noncovalent functionalization of small(cid:2)diameter nan(cid:2) otubes by specially synthesized chemical compound; • efficient enzyme immobilization on small(cid:2)diameter nonco(cid:2) valent functionalyzed nanotubes; application of conventional CCD matrix or photomultipliers for emission detecting. Innovative Aspect and Main Advantages An important advantage of the glucose and lactate sensors we propose to develop is the possibility of implanting the sensors directly into the tissue and using them for continuous monitor(cid:2) ing. Proposed glucose and lactate sensor will detect optical signal in the near infrared spectral region where human tis(cid:2) sues are optically transparent. Such a biosensor does not require outside power to operate and the information from the sensor can be obtained by means of wireless communication. Areas ooff Application Medicine, veterinary. 130 Fig.1 Schematical representation of the operation of the glucose biosensor Stage ooff Development The biosensor is under development. Influence of glucose level in aqueous solution on the nan(cid:2) otubes emission intensity was tested. It was found out that enzyme immobilized on DNA(cid:2)wrapped nanotubes keeps its native activity. Contact Details B.Verkin Institute for Low Temperature Phys&Engineering of the National Academy of Science of Ukraine Contact person: Dr. Karachevtsev Victor Alexeevich Head of Molecular biophysics department Address: 47, Lenin ave., 61103, Kharkiv, Ukraine. Tel.: +380 57(cid:2)340(cid:2)15(cid:2)95 Fax: +380 57(cid:2)340(cid:2)33(cid:2)70, 572(cid:2)333(cid:2)55(cid:2)93 E(cid:2)mail: karachevtsev@ilt.kharkov.ua