IDEA #4PDUH8 Functional Luminescent And Magnetic Nanoparticles As Bioanalytical Reagents And Drug Carriers

THE SCIENCE AND TECHNOLOGY CENTER IN UKRAINE Fig. 1. Transmission electron microscopy (××30,000): picture of colored functional polystyrene nanoparticles FUNCTIONAL LUMINESCENT AND MAGNETIC NANOPARTICLES AS BIOANALYTICAL REAGENTS AND DRUG CARRIERS Description Biocompatible colored, luminescent and magnetic nanoparticles with controlled particle size distribution, reactivity and functionality as well as the techniques of their synthesis were developed in collaboration with the Institute of Cell Biology of NASU, Ukraine, Lviv. We obtained a series of colloidal systems containing monodis(cid:28) perse nanoparticles with tailored particle morphology. Composite nanoparticles comprise of polystyrene, poly(cid:28) acrylate (including fluorine containing acrylates) or siliceous, Fe3O4, Ni, Ag core and functional reactive poly(cid:28) mer shell. The polymeric nanoparticles were sized 30, 100 and 300 nm. The size of hybrid polymer(cid:28)mineral nanopar(cid:28) ticles was 10–20 nm. Functional hybrid nanoparticles are surely protected from oxidation and contain functional shell providing targeted compatibility and dispersancy in various media. Functional nanoparticles of Ag, Fe3O4, and Ni, colored and luminescent polymeric nanoparticles that can bind specific proteins and interact with cell membrane, particularly with the apoptotic cells were developed. We successfully examined them as magnetic and luminescent markers, drug carriers and bioanalytical reagents. Stable reactive hydrosols of functional silver nanoparticles were tested for obtaining the hydrophobic coatings possessing antibacterial activity. We also developed micellar and col(cid:28) loidal polymeric drug nanocarriers and water based prepa(cid:28) rations with antimicrobial and fungicidal activities using physiologically active functional oligoperoxide surfac(cid:28) tants. They can be used as additives to the paints and var(cid:28) nishes providing the antimicrobial and fungicidal coating formation, for the sterilization of the working space, surgi(cid:28) cal instruments and materials. Some of these products were examined as remedies for plant protection from Mildew of cucumbers, Phytoftoros of tomatoes, and Grey rot of beans. Comparing with conventional materials the developed products are characterized by both the ability to chemical binding with various surfaces and prolongation of their action. The principal feature of the developed func(cid:28) tional nanoparticles is an availability of radical forming sites in their polymeric shell providing them an ability to initiate radical grafting and to introduce desired function(cid:28) al groups at definite distance from the particle core. Innovative Aspect and Main Advantages The development of polymeric and polymer(cid:28)mineral nanoparticles and their stable colloidal systems is based on using novel surface(cid:28)active coordinating metal complexes with functional oligoperoxide ligands as templates for the particle homogeneous nucleation, as initiators and stabiliz(cid:28) ers simultaneously. They determine nanoparticle size dis(cid:28) tribution and provide the formation of functional reactive polymeric shell irreversibly attached to the particle sur(cid:28) face. The opportunity to control conformational state of sorbed oligoperoxide molecules provides purposeful parti(cid:28) cle compatibility, the formation of stable colloidal systems in the media of various polarities and their desired rheo(cid:28) logical characteristics. The availability of peroxide, anhy(cid:28) dride, epoxy, isocyanate, carboxyl and other reactive func(cid:28) tional groups in polymeric shell allows grafting functional polymeric chains by radical reactions on the particle sur(cid:28) face and other polymer analogous transformations. Therefore, the developed functional nanoparticles are con(cid:28) venient universal products for the tailored introduction of various functional fragments on the definite distance from the surface providing targeted control of the particle spe(cid:28) cific properties and activity. Areas of Application Novel magnetic and colored polymeric functional nanocomposites can be used for the measuring the phago(cid:28) cytic activity of granulocytes of human blood, as carriers of immobilized enzymes and drugs.They can be used as addi(cid:28) tives to the paints and varnishes providing antimicrobial and fungicidal coating formation, for the sterilization of the working space, surgical instruments and materials. Some of these products were examined as remedies for plant protection from Mildew of cucumbers, Phytoftoros of tomatoes, and Grey rot of beans. Nanoparticles based on Ag, ferric oxide, Ni, and colored polymeric nanoparticles including luminescent ones can be used as specific markers for the detection and isolation of proteins, cells, including apoptotic cells. Stage of Development – The stage of the patenting. – Prototype available for testing Contact Details Lviv Politechnical National University Zaichenko Olexander zaichenk@polynet.lviv.ua; Phone +38 0322 582780 (work) Fax +38 0322 744300 (work) 46 SCIENCE AND INNOVATION. Special Issue, 2007