Fig. 1. ECL sensor module with accessories for assay accom%
plishment
THE SCIENCE AND TECHNOLOGY CENTER IN UKRAINE
ELECTROCHEMILUMINESCENT SENSOR FOR ASSAY OF BIOLIQUIDS
Description
The essence of our proposal is sensor aimed at assay of liq(cid:28)
uid mediums (in particular bioliquids) in medicine and
environmental monitoring. It is based on combination of
two main ideas: electrochemiluminescent (ECL) detection
method and sensor which electrodes inner surfaces are pre(cid:28)
pared on a base of nanotechnology approach by coating
with ordered molecular thickness films doped with elec(cid:28)
trochemiluminescers(cid:28)reactants.ECL assay method pos(cid:28)
sesses: i) high sensitivity due to optical detection of ana(cid:28)
lytical signal that allows reaching single(cid:28)photon detection
limit; ii) high selectivity of the assay due to two(cid:28)channel
registration mode, i.e. simultaneous recording of electro(cid:28)
chemical and optical (ECL) signals.Incorporation of elec(cid:28)
trochemiluminescer – reactant into film deposited onto
electrode allows using those reactants in mediums where
they are insoluble/unstable and thus broadening the list of
analytes that can be detected and mediums where the
assay can be done. The nanotechnology approach that is
used for thin films formation and their further deposition
onto working electrodes of the proposed ECL sensor is
Langmuir(cid:28)Blodgett method. It allows formation of uni(cid:28)
form ordered monomolecular films with highly control(cid:28)
lable predefined structure.The proposed sensor is aimed at
detection of certain aminoacids in bioliquids (urine and
blood) that is important for early recognition of metabolic
disorder and diseases accompanied by this disorder (e.g.
tryptophanurea).
Innovative Aspect and Main Advantages
Innovative aspect of the proposed technology consists in
combining analytical capabilities of liquid(cid:28)phase electro(cid:28)
chemiluminescence and the Langmuir(cid:28)Blodgett technolo(cid:28)
gy for electrochemiluminescers thin ordered layers forma(cid:28)
tion and deposition onto sensors' electrodes. This makes it
possible to utilize electrochemiluminescers as reactants,
which could not be used in bioliquids before, thus broad(cid:28)
ening the range of detected analytes important for biomed(cid:28)
ical and ecological applications.The detection of certain
aminoacids is possible due creation of new ECL composi(cid:28)
tion/ sensor structure that are capable of working in aque(cid:28)
ous systems.
Main advantages of the developed sensor are:
– low limit of detection (up to 2.5×10(cid:28)7 M);
– fast and simple assay protocols and sample preparation
(approx. 1–2 min., requires only one person);
– low sample volume (~20 μl);
– wide linear detection range 10(cid:28)7 (cid:28) 5×10(cid:28)3 M;
– fully automated assay procedure.
It is competitive with known analytical technologies for
bioliquids assay such as fluorescent, electrochemical etc.
Areas of Application
The developed sensor can be used for ECL assay of aque(cid:28)
ous mediums including bioliquids. In particular it can be
72
Contact Details
Contact persons: Dr. Sci., Prof. Mykola Rozhitskii,
Jun. Res. Yuriy Zholudov.
Lab. of Analytical Optochemotronics,
Dept of Biomedical Electronics,
Kharkiv National University of Radio Electronics,
14, Lenin Ave., Kharkiv, 61160, Ukraine
Phone: +38 057 7020369 Fax: +38 057 7021013
E(cid:28)mail: rzh@kture.kharkov.ua
Website: http://www.electrohim.kture.kharkov.ua
Fig. 2. Calibration curve of ECL sensor with model analytical
system. Linear detection range 10%7 % 5××10%3 M, limit of detec%
tion 2.5××10%7 M (S/N=3)
applied in:
– clinical assay;
– biological assay;
– environmental assay.
Other application areas can include pharmaceutical and
food products.
Stage of Development
Development phase – laboratory tested samples and assay
protocols are available.
SCIENCE AND INNOVATION. Special Issue, 2007
For more information or to license this innovation:
- Log In
to view the innovation's details
- Sign Up
with discount code "ECOS"