IDEA #53XE7G Electrochemiluminescent Sensor For Assay Of Bioliquids

Fig. 1. ECL sensor module with accessories for assay accom% plishment THE SCIENCE AND TECHNOLOGY CENTER IN UKRAINE ELECTROCHEMILUMINESCENT SENSOR FOR ASSAY OF BIOLIQUIDS Description The essence of our proposal is sensor aimed at assay of liq(cid:28) uid mediums (in particular bioliquids) in medicine and environmental monitoring. It is based on combination of two main ideas: electrochemiluminescent (ECL) detection method and sensor which electrodes inner surfaces are pre(cid:28) pared on a base of nanotechnology approach by coating with ordered molecular thickness films doped with elec(cid:28) trochemiluminescers(cid:28)reactants.ECL assay method pos(cid:28) sesses: i) high sensitivity due to optical detection of ana(cid:28) lytical signal that allows reaching single(cid:28)photon detection limit; ii) high selectivity of the assay due to two(cid:28)channel registration mode, i.e. simultaneous recording of electro(cid:28) chemical and optical (ECL) signals.Incorporation of elec(cid:28) trochemiluminescer – reactant into film deposited onto electrode allows using those reactants in mediums where they are insoluble/unstable and thus broadening the list of analytes that can be detected and mediums where the assay can be done. The nanotechnology approach that is used for thin films formation and their further deposition onto working electrodes of the proposed ECL sensor is Langmuir(cid:28)Blodgett method. It allows formation of uni(cid:28) form ordered monomolecular films with highly control(cid:28) lable predefined structure.The proposed sensor is aimed at detection of certain aminoacids in bioliquids (urine and blood) that is important for early recognition of metabolic disorder and diseases accompanied by this disorder (e.g. tryptophanurea). Innovative Aspect and Main Advantages Innovative aspect of the proposed technology consists in combining analytical capabilities of liquid(cid:28)phase electro(cid:28) chemiluminescence and the Langmuir(cid:28)Blodgett technolo(cid:28) gy for electrochemiluminescers thin ordered layers forma(cid:28) tion and deposition onto sensors' electrodes. This makes it possible to utilize electrochemiluminescers as reactants, which could not be used in bioliquids before, thus broad(cid:28) ening the range of detected analytes important for biomed(cid:28) ical and ecological applications.The detection of certain aminoacids is possible due creation of new ECL composi(cid:28) tion/ sensor structure that are capable of working in aque(cid:28) ous systems. Main advantages of the developed sensor are: – low limit of detection (up to 2.5×10(cid:28)7 M); – fast and simple assay protocols and sample preparation (approx. 1–2 min., requires only one person); – low sample volume (~20 μl); – wide linear detection range 10(cid:28)7 (cid:28) 5×10(cid:28)3 M; – fully automated assay procedure. It is competitive with known analytical technologies for bioliquids assay such as fluorescent, electrochemical etc. Areas of Application The developed sensor can be used for ECL assay of aque(cid:28) ous mediums including bioliquids. In particular it can be 72 Contact Details Contact persons: Dr. Sci., Prof. Mykola Rozhitskii, Jun. Res. Yuriy Zholudov. Lab. of Analytical Optochemotronics, Dept of Biomedical Electronics, Kharkiv National University of Radio Electronics, 14, Lenin Ave., Kharkiv, 61160, Ukraine Phone: +38 057 7020369 Fax: +38 057 7021013 E(cid:28)mail: rzh@kture.kharkov.ua Website: http://www.electrohim.kture.kharkov.ua Fig. 2. Calibration curve of ECL sensor with model analytical system. Linear detection range 10%7 % 5××10%3 M, limit of detec% tion 2.5××10%7 M (S/N=3) applied in: – clinical assay; – biological assay; – environmental assay. Other application areas can include pharmaceutical and food products. Stage of Development Development phase – laboratory tested samples and assay protocols are available. SCIENCE AND INNOVATION. Special Issue, 2007